Hoefer SE300 miniVE User Manual

user manualum SE300-IM/Rev.C0/06-12Hoefer SE300 miniVEElectrophoresis and Electrotransfer Unit

• p2Unpacking• Unwrap all packages carefully and compare contents with the packing list, making sure all items arrived.• If any part is missing, con

• p3Specifications Electrophoresis Gel sandwich size 10.0 cm wide × 8 to 10.5 cm longMax. tank volume 1.6 liters with one module in place 1.4 liter

• p4The Electrophoresis moduleThis section describes the use of the electropho-resis module. For instructions on using the blot module, see page 18.Th

• p5To open the module, swing the clamps outward. Lay the module flat on a work surface. Preparing self-cast gelsOne single gel can be cast on the m

• p6Place and seal the sandwich on the moduleTake care to “square” the three sealing sides of the sandwich. Hold the sandwich like a deck of cards an

• p7Turn each screw (alternating to keep the pressure even) until the clamps are loosely secured and will allow the spacers to be adjusted, if necess

• p8Lock the sealing plate into the closed position by engaging each tab in its topmost notch (Fig 9).Hang the module from the narrow side of the ta

• p9Warning!  Acrylamide is a neurotoxin. Always wear gloves and observe all laboratory safety procedures.Note: Approximately 10 ml of monomer solut

• p10Insert a comb (at a slight angle to prevent trapping air) into the sandwich, allowing the comb sides to rest on the spacers. Allow a minimum of

• p11Check that both gel surfaces will contact buffer. Check that the bottom gel-contact slot is exposed.Move the sealing plate into the “half open”

• piContents Important Information ... iiWaste Electrical and Electronic Equipment (WEEE) ...

• p12The minimum and maximum levels are marked. Verify that the lower electrode, which is approximately 2 cm from the bottom of the module, is complet

• p13Note: The amount of protein sample added to each well depends on both the sensitivity of the staining method and the distri-bution of protein am

• p14ElectrophoresisFor optimal resolution, start electrophoresis immediately after sample loading. Gels may be run at either constant current or cons

• p15Carefully lift the gel from the plate and lay it into a tray containing stain, fixative, or transfer buffer.Clean the unit as described in “Car

• p16Electrophoresis Troubleshooting problem solutionSmile effect on the buffer front To reduce the running temperature: • Fill the tank to the ma

• p17 problem solutionStained sample collects: Near the buffer front: • Protein is not sufficiently restricted by the resolving gel; increase the

• p18The blot module The Hoefer miniVE Blot Module, which can be ordered separately, performs electrotransfers on mini-format gels. Each module holds

• p19PreparationOptional: Passive coolingChill approximately 2 liters of deionized water to 4 °C. (Filling the tank with chilled water serves as a hea

• p20Prepare the transfer stackTransfer the sample as soon as possible after electrophoresis to minimize sample diffusion within the gel. Electrophore

• p21a. Center a packing sponge on the black cathode side.b. Lay one piece of wet filter paper on the sponge.c. Position the equilibrated gel on th

• piiImportant Information – English• If this equipment is used in a manner not speci-fied by Hoefer, Inc. the protection provided by the equipment m

• p22Check the position of the transfer stack. The transfer stack should be centered on the elec-trode plate. No layer should be pinched when the mod

• p23Important!  Buffer conductivity increases with increasing temperature, providing a positive feedback that results in rapid heating. We recommend

• p24problem possible cause solutionIncomplete transferBlank areas on the membrane Remove all trapped air bubbles in the transfer stack; take espe-cia

• p25Inefficient bindingChemical parametersFix or crosslink the mole to the requirements of the nucleic acid, protein, or membrane type. Prepare prote

• p26BibliographyPolyacrylamide gel electrophoresisAdams, L.D. and Gallagher, S.R. 1992. Two-Dimensional Gel Electrophoresis Using the O’Farrell Syste

• p27Ordering information product quantity code no.Hoefer SE300 miniVE, complete 1 SE300-10A-1.0 Includes Basic unit, 3 rectangular glass plate

• p28product quantity code no.Blotting accessories Sponge, Dacron packing 3/pk SE3005Blotting paper, 9 –10.5 cm 50/pk TE26 Gel casters Hoef

• p29Hoefer, Inc. 84 October Hill Road Holliston, MA 01746Toll Free: 1-800-227-4750 Phone: 1-508-893-8999 Fax: 1-508-893-0176 E-mail: support@hoeferin

• piiiBelangrijke Informatie – Dutch• Indien deze uitrusting in een manier wordt gebruikt die niet door Hoefer, Inc. is gespecificeerd de bescherming

• pivl’instrument. Les dissolvants organiques causeront des dommages irréparables à l’unité!• Ne pas fonctionner avec les températures de tampon au-d

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• pvipor Hoefer, Inc. puede ser utilizado para operar, para mantener, y para atender a este producto.• Sólo utiliza una alimentación que es CE marcó

• pviiEnglishFrenchGermanItalianSpanishSwedishThis symbol indicates that the waste of electrical and electronic equipment must not be disposed as unso

• p1IntroductionThe Hoefer® SE300 miniVE vertical electropho-resis system performs vertical gel electrophoresis on mini-format gels. The basic unit in