Hoefer HE33 User Manual download pdf (Page 11)

•

Operating instructions

Agarose gels are ﬁrst prepared using the gel cast-

ing kit. Samples are then loaded into wells and

electrophoretically separated. The ﬂuorescent

dye ethidium bromide can be added to the gel or

electrophoresis buffer or both to track separa-

tion progress. After electrophoresis, the gel may

be stained and photographed, blotted, or dried

for autoradiography.

Fill the base with coolant

Even if no cooling is required, it is important

to ﬁll the base with the proper coolant solution

before the ﬁrst use because the solution provides

a necessary heat sink.



Prepare 600 ml of 50/50 ethylene glycol/water.

Optional: To help see wells more clearly while loading

the sample, add a drop or two of soluble dye or food

color to the coolant solution.

Locate the two inlet holes in the top edge of the base.

Fill the base cavity as full as possible with coolant

using a 50-ml syringe or a pump.



Push a gray rubber plug into each hole, taking care

that the plug is securely seated.



Place the prepared base in an ice bucket or into a

refrigerator or freezer set no lower than -20 °C for

about an hour before use. (The base will always be

ready if you store it in the refrigerator or freezer.)

Important! Do not ﬁll the base

with commercial antifreeze,

organic solvents, or pure water.

Note: It is not necessary to

replace the coolant.

1 2 ... 6 7 8 9 10 11 12 13 14 15 16 ... 25 26

No comments

Hoefer HE33 User Manual Page 11