Hoefer HE33 User Manual download pdf (Page 13)

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Cast the gel



Install the running tray

Firmly grasp the casting tray with one hand. With

the other hand, place one end of the running tray

against the foam pad at the bottom edge, press the

tray against the pad, and then lower it to rest on the

bottom of the casting tray, seating the other end of

the tray against the opposite foam pad.



Prepare the comb(s)

Fit the two slots in the comb between the (loosened)

thumb screw heads and the comb back. Tighten the

screws until the comb is just supported. Seat the

comb assembly on the rim of the casting tray and

adjust the bottom of the comb so that it is about

1.0 mm from the running tray. Tighten the screws

to secure the comb. To run twice as many samples,

prepare two combs.



Remove the comb assembly. Place the casting

assembly on a leveling surface and level, using the

spirit level on the running tray as a guide.



Pour the agarose solution (cooled to 50 °C) into the

casting tray. Orient the comb assembly so that the

comb faces the nearest foam pad and seat it on the

tray rim. Check that the comb is vertical to prevent

well shape distortions. To run twice as many samples,

place the second comb assembly in the center of

the tray. Allow a minimum of 30 minutes for the gel

to set.

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Hoefer HE33 User Manual Page 13